This might look a very silly question to many of you but please answer.
I am interested in generating a volcano plot for my dataset, which has four columns and all values are in log2. column 1 has name while column 2, 3, 4 has values in treated, untreated states and fold change.
DDR1 7.8519007358 8.0207450402 0.1688443044 RFC2 8.1756591822 7.7784602732 -0.397198909 HSPA6 7.8186806878 7.274639204 -0.5440414838 PAX8 8.0207450402 3.8519007358 -4.1688443044 CALR 7.7784602732 1.8519007358 -5.9265595374 MAPK1 4.8519007358 7.7784602732 2.9265595374 MAPK1 4.8819007358 7.2784602732 2.3965595374 KRAS 3.8519007358 7.8519007358 4
I am intrested in generating scatter plot from these values with marking outliers at 2 fold.
I am bit confused and puzzled with the command in R as it is asking more than one parameter like p-value and things which I do not have.
Kindly help me in generating this scatterplot with my dataset.
Solutions in python are also welcome.
Thank you for your time and consideration.