Disclaimer: Statistics is not exactly something I am particularly good at. So if this is a stupid question, I do apologize
I have been using non-linear regression to analyze binding between two biomolecules. Each individual experiment was done in triplicate. The average of these triplicate values (with associated error) were analyzed using regression ([L]=known).
Specific binding = Bmax*[L]/(Kd+[L])
The Bmax and Kd are calculated. Both values have 95% confidence intervals and standard errors reported.
I would like to compare the Kd for different pairs of Biomolecules.
I.e. I would like to compare the Kd (higher or lower) for A binding to B, A binding to C and A binding to D.
In addition, I would like to compare binding between AB and XY.
Which test(s) would be appropriate for this analysis?
Any suggestions would be greatly appreciated!!