How to estimate the out crosses rate in crops? in have experiment included; main plot (1- seeds from open field 2- seeds from free pollinators cage 3-seeds from pollinator cage), sub plot (5 genotypes) and 50 plants grown for each genotype in each plot. This in three replications. It easy to estimate variance between main plots and sub plots but my question about the genetic variance between plants in same plot. and this will be due to out cross.