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Aug 25, 2023 at 14:51 comment added dipetkov Thank you so much for responding and congratulations on publishing your work in Nat Sci Reports! I took a look at the paper and I think the interesting structure in the data (what I called "clusters of three") is explained by the design of the experiment and that there were 15 subsamples analyzed. I admit though that I didn't understand lots of the science as I have no knowledge of sequencing. Again, thanks for sharing the extra information!
Aug 25, 2023 at 0:01 comment added teaelleceecee @dipetkov each data point is an independent measurement as there were three technical replicate samples per viral spike level, so each of these were measured separately, not repeatedly (I hope I interpreted your question correctly). I ended up publishing this work: nature.com/articles/s41598-019-55741-3 Figure 1 illustrates the experiment design, which might help in understanding what the data actually represent and how it was collected.
Aug 20, 2023 at 16:47 comment added dipetkov 4 years later I have a followup question. (This is the first time I come across this thread.) I notice the observations come in clusters of three with about the same x. Are these data points independent measurements? Or -- and I think this is more likely -- are they replicate measurements taken during the same lab experiment? All the answers so far assume the data consists of 30 independent data points, not 10 independent experiments (5 for each virus) with three repeat measurements each.
Sep 12, 2019 at 13:12 history edited kjetil b halvorsen
edited tags
Jul 26, 2019 at 3:32 vote accept teaelleceecee
Jul 25, 2019 at 16:02 history protected gung - Reinstate Monica
Jul 25, 2019 at 15:55 answer added Sam Mason timeline score: 10
Jul 23, 2019 at 18:56 answer added Carl Witthoft timeline score: 4
Jul 23, 2019 at 13:07 comment added Carl Witthoft You don't have enough data points in the transition region to claim with any authority that there's a smooth curve. I could just as easily fit a Heaviside function to the points you are showing us.
Jul 23, 2019 at 11:55 comment added jochen I agree with your supervisor, fitting a sigmoidal curve would be a good choice. The two lines you show in your second plot seem not to be sigmoidal? Have a look on Wikipedia .
Jul 23, 2019 at 6:00 history tweeted twitter.com/StackStats/status/1153545329129734147
Jul 22, 2019 at 20:19 comment added James Phillips After your edit to include the actual data, I have updated my answer with a plot of the 3-parameter logistic type equation using your updated data.
Jul 22, 2019 at 20:12 comment added Ben Bolker PS I would encourage you not to suppress standard errors with se=FALSE. Always nice to show people how large the uncertainty actually is ...
Jul 22, 2019 at 20:10 vote accept teaelleceecee
Jul 26, 2019 at 3:32
Jul 22, 2019 at 20:06 comment added teaelleceecee @BenBolker yes, this removed the extended arch on the curves - thank you!
Jul 22, 2019 at 19:41 comment added Ben Bolker try adding method.args=list(family=quasibinomial)) in the arguments to geom_smooth() in your original ggplot code.
Jul 22, 2019 at 18:48 answer added Ed V timeline score: 4
Jul 22, 2019 at 18:34 history became hot network question
Jul 22, 2019 at 17:48 answer added Nick Cox timeline score: 14
Jul 22, 2019 at 17:25 answer added Aksakal timeline score: 4
Jul 22, 2019 at 17:14 history edited teaelleceecee CC BY-SA 4.0
Added data
Jul 22, 2019 at 16:33 history edited mkt CC BY-SA 4.0
Edited for clarity
Jul 22, 2019 at 16:20 answer added James Phillips timeline score: 4
Jul 22, 2019 at 15:31 answer added mkt timeline score: 17
Jul 22, 2019 at 14:28 comment added user158565 Try (2) piece-wise (linear) model.
Jul 22, 2019 at 11:03 comment added mkt Seems like a logistic regression would be best, since this is bounded between 0 and 100%.
Jul 22, 2019 at 10:41 comment added Roland Make use of the "G" in "GAM". I don't know enough about genome coverage (appears to be a percentage?) to recommend a family but you probably want a logit link.
Jul 22, 2019 at 10:30 review First posts
Jul 22, 2019 at 11:29
Jul 22, 2019 at 10:27 history asked teaelleceecee CC BY-SA 4.0