I want to test if the means of different groups (tissue types) are different using one-way ANOVA. Most of the groups have replicates but there is one group that only has one sample. Like this:
heart, heart, heart, skin, skin, skin,skin, bone, bone, bone, brain
In the above example, only one sample/data point for brain and the other three tissues have replicates.
So can ANOVA work for this data or it has to require all groups have replicates?