Mixing chi-squared and fisher's exact tests I'm trying to look across a dozen or so groups of cases with respect to a condition they may have or not. I have a predetermined expected frequency for each condition status (c1 & c2, let's assume yes/no)
Now, I'm performing a simple test of significance between the observed and expected values per group as if each were a 2x2 contingency table. In cases where all values are above 5, I perform a chi-square test and all other cases I perform Fisher's exact test of significance.
Is it incorrect to perform different tests per group? I'm not necessarily interested in comparing between groups but rather trying to determine if the difference between c1 and c2 is significant from what would be expected in each condition per group.
Would it make more sense just to use Fisher's exact test on everything?
Here's a mock representation of my data. group1 and 3 use Fisher's test as there are some values below 5, while groups 2 and 4 use Chi-square.
        c1.obs  c2.obs  c1.exp  c2.exp  p.value
group1  3       21      2       22      1
group2  9       48      7       60      5.5E-7
group3  13      19      3       29      8.1E-03
group4  34      182     22      194     4.9E-03 
...

 A: As far as I can tell from the comments on the original post, analyzing a 2 x 2 contingency table doesn't make sense for these data.  Instead you would want to compare the count of c1 out of the total observed count, and compare that to 0.10.  
You might use an exact binomial test or a chi-square test goodness-of-fit test.  So in R, for group 3:
yes = 13
no  = 19
binom.test(yes, (yes+no), 0.1)

   ### number of successes = 13, number of trials = 32, p-value = 5.507e-06
   ### alternative hypothesis: true probability of success is not equal to 0.1
   ### 95 percent confidence interval:
   ###  0.2369841 0.5935508
   ### sample estimates:
   ### probability of success 
   ###                0.40625 

yes = 13
no  = 19
chisq.test(c(yes, no), p=c(.10, .90))

   ### Chi-squared test for given probabilities
   ### 
   ### X-squared = 33.347, df = 1, p-value = 7.709e-09

A: As far as I know you can use chi-square throughout, unless the expected counts are less than five in which case you can use Fisher's exact test.  I'm not sure but I think Fisher's Exact Test is slightly more conservative in its resulting p-value, i.e., it's slightly less likely to show statistical significance (but I'm not sure of that!)  Some texts say that if any expected frequencies are less than 2, or if more than 20% of the expected frequencies are <5, then don't use chi-square but it's okay to use it otherwise.  
You can also combine cells or collapse categories together in your tables if this is possible, to raise the expected frequencies to greater than 5.  
