Image shows sample values: bac- Bacteria, prop- Properties
I am studying 300 bacteria sequenced from same sample.
They are grouped in 7 groups having varying number of members. e.g. some group has only 2 while some group has 200. I am comparing the gene properties of those groups. e.g. number of genes doing some required function.
How to normalize these properties (observations are values ranging from 1 to 100) for such uneven group sizes for proper comparison (similarity or difference) with statistical significance.
I just want to compare which properties are significantly high or low in a particular group. But due to uneven group sizes I can see the values are gonna be high in larger groups while values are low for small groups.
The values from a group of 2 members may have values like 2, 4, or any small number and for groups of 100 or more the values will cross 50 to 100. So may I simply divide the values with number of members of the group? or there is any better method.