A probabilistic Structural Causal Model (SCM) is defined as a tuple $M = \langle U, V, F, P(U) \rangle$ where $U$ is a set of exogeneous variables, $V$ a set of endogenous variables, $F$ is a set of structural equations that determines the values of each endogenous variable and $P(U)$ a probability distribution over the domain of $U$.
In a SCM we represent the effect of an intervention on a variable $X$ by a submodel $M_x = \langle U, V, F_x, P(U) \rangle$ where $F_x$ indicates that the structural equation for $X$ is replaced by the new interventional equation. For example, the atomic intervention of setting the variable $X$ to a specific value $x$ --- usually denoted by $do(X = x)$ --- consists of replacing the equation for $X$ with the equation $X = x$.
To make ideas clear, imagine a nonparametric structural causal model $M$ defined by the following structural equations:
$$
Z = U_z\\
X = f(Z, U_x)\\
Y = g(X,Z, U_y)
$$
Where the disturbances $U$ have some probability distribution $P(U)$. This induces a probability distribution over the endogenous variables $P_M(Y, Z, X)$, and in particular a conditional distribution of $Y$ given $X$, $P_M(Y|X)$.
But notice $P_M(Y|X)$ is the "observational" distribution of $Y$ given $X$ in the context of model $M$. What would be the effect on the distribution of $Y$ if we intervened on $X$ setting it to $x$? This is nothing more than the probability distribution of $Y$ induced by the modified model $M_x$:
$$
Z = U_z\\
X = x\\
Y = g(X, Z, U_y)
$$
That is, the interventional probability of $Y$ if we set $X= x$ is given by the the probability induced in submodel $M_x$, that is, $P_{M_x}(Y|X=x)$ and it's usually denoted by $P(Y|do(X = x))$. The $do(X= x)$ operator makes it clear we are computing the probability of $Y$ in a submodel where there is an intervention setting $X$ equal to $x$, which corresponds to overriding the structural equation of $X$ with the equation $X =x$.
The goal of many analyses is to find how to express the interventional distribution $P(Y|do(X))$ in terms of the joint probability of the observational (pre-intervention) distribution.
do-calculus
The do-calculus is not the same thing as the $do(\cdot)$ operator. The do-calculus consists of three inference rules to help "massage" the post-intervention probability distribution and get $P(Y|do(X))$ in terms of the observational (pre-intervention) distribution. Hence, instead of doing derivations by hand, such as in this question, you can let an algorithm perform the derivations and automatically give you a nonparametric expression for identifying your causal query of interest (and the do-calculus is complete for recursive nonparametric structural causal models).