Well the short answer is yes, you can calculate the p.value for your estimate...
However, the main problem with using such a small sample is that it would be difficult to generalise such a finding to your given target population, assuming it is larger and more diverse than your sample of 25 individuals. If random sampling were used, how likely would it be for the sample to be representative of your target population? One outlier or uncommon observation could have a significant impact upon your estimates, or a subgroup may be over represented. This needs to be considered throughout the study.
If you are estimating parameters of your model using OLS, a larger sample size generally provides an estimate closer to the true value of the parameter of interest. This is due to the consistency of the OLS estimator.
If $$n_{i}$$ represents the number of observations in a sample and
$$n_{1}<n_{2}<n_{3}$$

(Wooldridge, 2006)
You may get an accurate estimate for the sample of 25, but whether that sample it representative of your target population is another matter. It should be noted that the OLS estimator is not biased in cases where a small sample is present. It is the precision of the estimate which increases with the sample size. Though there is no guarantee that the true parameter lies within your given confidence interval for an individual sample, this increase in precision is useful in justifying subsequent inference.
There is also much debate as to the usefulness of p.values in general, and in many cases it is preferable to present confidence intervals as well/instead (Sterne and Davey Smith, 2001). Such statistics should be interpreted within the context of previous theory and study design elements. Essentially, use it as part of your interpretation, but not necessarily as the basis of your interpretation.
I hope that is of some help :)
References
Sterne J, Davey Smith G., 2001, “Sifting the evidence - what's wrong with significance tests?” Physical Therapy vol.81, pp.1464-1469 (reprint of article 249) and the American Statistical Association statement and the 21 supporting papers)
Wooldridge, J. M. 2006. Introductory econometrics: a modern approach. Mason, OH, Thomson/South-Western.