I would like to know what kind of test I can use to verify my findings. In general I have a two group of people. One is a control group the other one was treated with a drug. After some period of time we collected samples from them (urine, blood, spit, etc...) and we calculated the ratio between some metabolites which can be found in those specific samples. The ratio was calculated for the metabolites grouped within the samples they were collected. For example:
Control: Treatment: Ratio: Urine (Arginine) / Urine (Arginine) 1.5 Urine (Proline) / Urine (Proline) 1.2 Urine (Acid) / Urine (Acid) 1.3 Blood (Tryptophane) / Blood (Tryptophane) 1.7 Blood (XX1) / Blood (XX1) 1.3 Blood (XX2) / Blood (XX2) 1.4
So I would like to know if a whole group (Blood, Urine, Spit) was significantly changed. I have at least 4 replicates for each of the metabolite/group. I would like to store all the ratios within replicates and perform a test which will tell me if the ratio is significantly changed to the one of the sides. What kind of a test can be used ?