I have measured nitrogen release from sediment in a river at six different sites (blue squares), at each site I took 3 replicate measurements of nitrogen release within each of two habitat types (areas with and without plants, green and grey circles). Sites differed in underlying sediment type, either Sandy or Muddy.
The design is unbalanced, 2 sites have muddy sediment and four have sandy.
I am trying to assess the effect of habitat type, site and sediment type (an their interactions) on nitrogen release.
My understanding is that this is a hierarchical or nested design, with habitat type nested within site, which is nested within sediment type. Is this correct?
Would be grateful for any help on this or point me to some reading!