I'm working on cancer mutation analysis from system perspective and ran across a statistical problem. My research subject is "network motif" in cancer. In my case, there are 53 different motifs and each of them has two kinds of mutations (i.e. pathogenic or neutral) with a normalized count.
Row names are motif IDs, third column is ratio of pathogenic/neutral.
My task is to determine a threshold of this ratio (e.g. 2). And I want to say that any motif whose ratio above this threshold indicates that the difference between pathogenic and neutral mutations on this motif is statistically significant.
I would appreciate help with choosing a statistical test for this problem.