I performed an experiment where I raised different families coming from two different source populations. Each family was assigned one of two treatments. After the experiment I measured several traits on each individual. To test for an effect of either treatment or source as well as their interaction, I used a linear mixed effect model with family as random factor, i.e.
so far so good, Now I have to calculate the relative variance components, i.e. the percentage of variation that is explained by either treatment or source as well as the interaction.
Without a random effect, I could easily use the sums of squares (SS) to calculate the variance explained by each factor. But for a mixed model (with ML estimation), there are no SS, hence I thought I could use Treatment and Source as random effects too to estimate the variance, i.e.
However, in some cases, lme does not converge, hence I used lmer from the lme4 package:
Where I extract the variances from the model using the summary function:
model<-lmer(Trait~1+(Treatment*Source|Family),data=regrexpdat) results<-VarCorr(model) variances<-results[,3]
I get the same values as with the VarCorr function. I use then these values to calculate the actual percentage of variation taking the sum as the total variation.
Where I am struggling is with the interpretation of the results from the initial lme model (with treatment and source as fixed effects) and the random model to estimate the variance components (with treatment and source as random effect). I find in most cases that the percentage of variance explained by each factor does not correspond to the significance of the fixed effect.
For example for the trait HD, The initial lme suggests a tendency for the interaction as well as a significance for Treatment. Using a backward procedure, I find that Treatment has a close to significant tendency. However, estimating variance components, I find that Source has the highest variance, making up to 26.7% of the total variance.
anova(lme(fixed=HD~as.factor(Treatment)*as.factor(Source),random=~1|as.factor(Family),method="ML",data=test),type="m") numDF denDF F-value p-value (Intercept) 1 426 0.044523 0.8330 as.factor(Treatment) 1 426 5.935189 0.0153 as.factor(Source) 1 11 0.042662 0.8401 as.factor(Treatment):as.factor(Source) 1 426 3.754112 0.0533
And the lmer:
summary(lmer(HD~1+(as.factor(Treatment)*as.factor(Source)|Family),data=regrexpdat)) Linear mixed model fit by REML Formula: HD ~ 1 + (as.factor(Treatment) * as.factor(Source) | Family) Data: regrexpdat AIC BIC logLik deviance REMLdev -103.5 -54.43 63.75 -132.5 -127.5 Random effects: Groups Name Variance Std.Dev. Corr Family (Intercept) 0.0113276 0.106431 as.factor(Treatment) 0.0063710 0.079819 0.405 as.factor(Source) 0.0235294 0.153393 -0.134 -0.157 as.factor(Treatment)L:as.factor(Source) 0.0076353 0.087380 -0.578 -0.589 -0.585 Residual 0.0394610 0.198648 Number of obs: 441, groups: Family, 13 Fixed effects: Estimate Std. Error t value (Intercept) -0.02740 0.03237 -0.846
Hence my question is, is it correct what I am doing? Or should I use another way to estimate the amount of variance explained by each factor (i.e. Treatment, Source and their interaction). For example, would the effect sizes be a more appropriate way to go?