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Premise: not very clever in statistics!

Data: I have quantitative data (two variables, A and B) on two small groups of subjects (both N=7); I'm going to perform a T-test to check differences about those groups. Before it, I run a normality test on data A and B of first group, using Matlab lillietest function. The test says A is normal (H0 holds), B is not (H0 rejected). Here are my data, followed by p values:

A       B    
0.000   0.125
1.500   0.125
2.375   1.125
2.375   0.125
5.625   0.250
4.250   0.000
0.750   0.000

p=0.37  p=0.008
H0=1    H0=0

I obtained similar results for second group. My conclusion is that I can perform t-test only for variable A, not for B.

Question 1) is my conclusion correct?

Question 2): given the very small dimension of sample size (n=7), how should I consider the normality test responses? Did I run a meaningful test? Is there a minimum sample size for it?

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Elaboration on t.f's answer.

The normality test is a sneaky beast, because conceptually it works the other way round than a "normal" statistical test. Normally, you base your knowledge based on the rejection of the null. Here, the "desired" outcome ("proof" of normality) is the non-rejection. However, failure to reject is not the same as proving the null! The fact that cannot find an effect, does not mean it is not there.

With few samples, you will therefore never reject your hypothesis, so you are likely to falsely assume that your data is normal.

Conversely, if you have plenty of data, you will always reject your null, because no data in real world is perfectly normal. Consider human height - typically assumed, in biology, to have a normal distribution. In fact, it has been assumed to be normal for the past 150 years (ever since Galton). However, height has clear boundaries: it cannot be negative, it cannot be 100 meters. Therefore, it cannot be normally distributed.

You will find a more detailed discussion with numerous examples here.

So what can you do?

  • Is there any reason to believe that your data is not normally distributed? Can you guess the distribution a priori? Typical examples may include bacterial growth or frequency of occurrence of an event.
  • Use a q-q plot or a similar visual aid to make the decision.
  • If forced by your thesis advisor to make a normality test, rather than focusing on the p-value alone, consider the effect size or calculate the skewness.
  • Do you have similar data from other experiments? Can you use it to increase your sample size?
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    $\begingroup$ I got the point of @January "With few samples, you will never reject your hypothesis(...)". The reason I wanted to test normality is that I'm going to perform a t-test (for a scientific article to be submitted); unfortunately I've no idea if population is normal (no data about it); It is not clear to me if I can run anyway the t-test as it is, as stated by t.f "(...) fairly robust to normality violations". My concerns are about possible criticism from reviewers; to be honest, I often read articles with t-test on small samples, and no mention to normality test... maybe I'm too fussy. $\endgroup$ – valerio_sperati Jan 24 '18 at 17:10
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    $\begingroup$ I think that plotting with only 7 observations is problematic, as well as asking if there is a reason for the the data to be normally distributed. I think the better question is, is there any reason to believe that the data is distributed normally (or at least symmetrically (which is usually enough)). Beside that all the points are usually good to follow $\endgroup$ – Kozolovska Jan 24 '18 at 19:27
  • $\begingroup$ You say :Normally, you base your knowledge based on the rejection of the null. Here, the "desired" outcome ("proof" of normality) is the non-rejection." That is, we should do normality testing as equivalence testing. Any references studying normality testing that way? $\endgroup$ – kjetil b halvorsen Oct 10 '18 at 19:18
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  1. Your conclusion is correct, the normality assumption is required for t-test. However the t-test is fairly robust to violations of it. In any case you can use a non parametric for example the test Mann Whitney when you can guarantee the normality assumption.

  2. You should, with such a small amount of observation the power of your test is small, and therefore the 'effect' is probably large if you detected it (meaning the data is very not normal). It usually the problem when you have thousand of observation you reject the hypothesis that the distribution is normal but it usually due to the fact that nothing is perfectly normal and you have a lot of power even when the 'effect' is weak.

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