I was wondering if anyone had any advice on comparing two groups where the variance in once group is high and the variance in the other group is low.
Specifically, I am attempting differential gene expression analysis using EdgeR. I have been using the glmLRT function however while playing with other functions I discovered the BCV was 0.79. My understanding is that using a generalised linear model is probably not a valid method to compare these two groups (each with 5 replicates). Can anyone offer advice?