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In my lab I need to test if there's an significance between male and female sexual dimorphism on body temperature over 40 minutes. The temperature is simulated with a heat lamp and is turned on at 0 minutes and stays the same for the duration of the experiment. I've done multiple trials, and for each trial there's 9 data points collected (temperature collected from male and female every 5 minutes). Is there a model I can fit in R, and a significance test (T-test?) that would fit best for my data? Should I do a permutation test?

Thank you! enter image description here

(Green: Female, Blue: Male)

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You should log-transform the temperature to try to linearize it and then do an ANCOVA of the form log(Temperature) ~ Time + Sex.

You might have to try log-transorming time as well, if the log-transformation of temperature alone is not enough to achieve linearity on the temperature-time graph for each sex.

See more info and examples here.

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  • $\begingroup$ That might work well, thank you so much! I will look more into it. $\endgroup$ – Michael Lee Apr 29 at 18:49

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