Using only the most basic axioms about probabilities and real numbers, one can prove a much stronger statement:
The difference of any two independent, identically distributed nonconstant random values $X-Y$ never has a discrete uniform distribution.
(An analogous statement for continuous variables is proven at Uniform PDF of the difference of two r.v.)
The idea is that the chance $X-Y$ is an extreme value must be less than the chance that $X-Y$ is zero, because there is only one way to (say) maximize $X-Y$ whereas there are many ways to make the difference zero, because $X$ and $Y$ have the same distribution and therefore can equal each other. Here are the details.
First observe that the hypothetical two variables $X$ and $Y$ in question can each attain only a finite number $n$ of values with positive probability, because there will be at least $n$ distinct differences and a uniform distribution assigns them all equal probability. If $n$ is infinite, then so would be the number of possible differences having positive, equal probability, whence the sum of their chances would be infinite, which is impossible.
Next, since the number of differences is finite, there will be a largest among them. The largest difference can be achieved only when subtracting the smallest value of $Y$--let's call it $m$ and suppose it has probability $q = \Pr(Y=m)$--from the largest value of $X$--let's call that that one $M$ with $p = \Pr(X=M).$ Because $X$ and $Y$ are independent, the chance of this difference is the product of these chances,
$$\Pr(X-Y = M - m) = \Pr(X=M)\Pr(Y=m) = pq \gt 0.\tag{*}$$
Finally, because $X$ and $Y$ have the same distribution, there are many ways their differences can produce the value $0.$ Among these ways are the cases where $X=Y=m$ and $X=Y=M.$ Because this distribution is nonconstant, $m$ differs from $M.$ That shows those two cases are disjoint events and therefore they must contribute at least an amount $p^2 + q^2$ to the chance that $X-Y$ is zero; that is,
$$\Pr(X-Y=0) \ge \Pr(X=Y=m) + \Pr(X=Y=M) = p^2 + q^2.$$
Since squares of numbers are not negative, $0 \le (p-q)^2,$ whence we deduce from $(*)$ that
$$\Pr(X-Y=M-m)=pq \le pq + (p-q)^2 = p^2 + q^2 - pq \lt p^2 + q^2 \le \Pr(X-Y=0),$$
showing the distribution of $X-Y$ is not uniform, QED.
Edit in response to a comment
A similar analysis of the absolute differences $|X-Y|$ observes that because $X$ and $Y$ have the same distribution, $m=-M.$ This requires us to study $\Pr(X-Y=|M-m|) = 2pq.$ The same algebraic technique yields almost the same result, but there is the possibility that $2pq=2pq+(p-q)^2$ and $2pq+p^2+q^2=1.$ That system of equations has the unique solution $p=q=1/2$ corresponding to a fair coin (a "two-sided die"). Apart from this exception the result for the absolute differences is the same as that for the differences, and for the same underlying reasons already given: namely, the absolute differences of two iid random variables cannot be uniformly distributed whenever there are more than two distinct differences with positive probability.
(end of edit)
Let's apply this result to the question, which asks about something a little more complex.
Model each independent roll of the die (which might be an unfair die) with a random variable $X_i,$ $i=1, 2, \ldots, n.$ The differences observed in these $n$ rolls are the numbers $\Delta X_i = X_{i+1}-X_i.$ We might wonder how uniformly distributed these $n-1$ numbers are. That's really a question about the statistical expectations: what is the expected number of $\Delta X_i$ that are equal to zero, for instance? What is the expected number of $\Delta X_i$ equal to $-1$? Etc., etc.
The problematic aspect of this question is that the $\Delta X_i$ are not independent: for instance, $\Delta X_1 = X_2-X_1$ and $\Delta X_2 = X_3 - X_2$ involve the same roll $X_2.$
However, this isn't really a difficulty. Since statistical expectation is additive and all differences have the same distribution, if we pick any possible value $k$ of the differences, the expected number of times the difference equals $k$ in the entire sequence of $n$ rolls is just $n-1$ times the expected number of times the difference equals $k$ in a single step of the process. That single-step expectation is $\Pr(\Delta X_i = k)$ (for any $i$). These expectations will be the same for all $k$ (that is, uniform) if and only if they are the same for a single $\Delta X_i.$ But we have seen that no $\Delta X_i$ has a uniform distribution, even when the die might be biased. Thus, even in this weaker sense of expected frequencies, the differences of the rolls are not uniform.