I have done a Western blot experiment and I am trying to figure out the best statistical test to perform on my data. I am comparing the differences in the expression levels of a certain protein in the brains of wild-type mice from two different age groups (postnatal day 12 and postnatal day 30). I have 10 samples in the postnatal day 12 group and 9 samples in the postnatal day 30 group.
I have performed an unpaired parametric t-test to see if there is a statistically significant difference in the protein expression levels between the two age groups. I have performed an unpaired t-test because evidently the mice in the two age groups are different from each other.
However, within each age group, there are some technical replicates (e.g. samples from the same mouse that were used in repeats of the experiment to see if the observed effect is reproducible). I was wondering if it is suitable to use an unpaired t-test if there are technical replicates of a sample within an experimental group?
Any insights are appreciated.