I have a 2X2 factorial experiment where I am interested in seeing the effect of two different nutrient solutions (N and W) on the appearance of root tips of two different plant species (A and B). I used 3 plants per species X treatment combination. I took daily counts of the number of root tips of each plant over the course of 9 days. The data look like this.
I have never worked with discrete data before. I was thinking of fitting an exponential growth function such as:
, where T0 is the number of tips at time 0 (the observed initial number of tips), r is the relative rate of addition of new tips and t is time (in days, considered continuous?), and see if there were differences in the r parameter between my species X treatment combinations (or if there are only main effects…). Any suggestions? I know I will have to account for issues such as correlated observations and having (random?) subject-specific effects on the model parameters. I have no idea how to do this in the context of discrete data analysis, and would really appreciate any suggestions of potential models to use, pitfalls and references that would give me some background.