I am performing a temperature preference test in mice. Mice are placed into a chamber with one half of a chamber having a cold floor and the other half having a warm floor. Mice spend a specific total time in the chamber (3 min) and can select the cold or warm part of the chamber. I calculate the time (in percent of the total time) spent on each side for each subject (each mouse). The results look like this:
Mouse Warm% Cold% 1 85% 15% 2 80% 20% 3 87% 13% 4 84% 16%
How do I calculate the confidence interval for the mean cold time percentage of the population from which this group of mice was selected?
If I perform the same test with a second group of mice which received some experimental treatment, which statistical test to use to check for a difference between two groups of mice?