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So I'm trying to see the effect of 2 different types of essential oils on e.coli (Grade 12 student - this is for my IB Biology EE)

What I did was that I increased the concentrations of the oils from 10% to 20%, 30%, 40%, and 50%. I used the disk diffusion tests and measured the inhibition zones formed. I also had a positive control being an antibiotic so I compare with that too. In addition to that I combined the two oils in 5 different ratios (1:5, 2:4, 3:3, 4:2, 5:1) to see if there's a synergistic effect. So if I'm not wrong, I've got 2 independent variables and 1 dependent variable (not sure if the combination ratios are variables too somewhere?).

Based off of what I've read so far, there are one-way and two-way ANOVA tests, as well as t-tests, and Tukey's HSD test. I cannot tell which are the most relevant in my case. I also have no idea what method to use for measuring synergy.

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    $\begingroup$ Hello and welcome to CrossValidate. Can you provide a dummy table/sample of your data? $\endgroup$
    – Derf
    Commented Feb 18 at 12:36
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    $\begingroup$ Might also help if you add what's your academic background and level so that response can be catered to your needs. $\endgroup$
    – Derf
    Commented Feb 18 at 13:27
  • $\begingroup$ I have not yet finished tabulating the data, but once I do I might be able to provide a dummy table. Do not know how long that will take since I'm quite slow, but I'd appreciate if I could get some general idea as to how I can approach this. $\endgroup$ Commented Feb 18 at 13:40
  • $\begingroup$ How do you measure effect? Survival? $\endgroup$ Commented Feb 18 at 14:18
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    $\begingroup$ Please add that new information as an edit to the post! We want posts to be self-contained, and comments on this site are often seen by very few people, and can be removed $\endgroup$ Commented Feb 19 at 13:56

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