I am measuring hormone changes in animals. I have three groups: Groups A, B, and C. In each group, blood was collected from the SAME individuals for hormone assay at 0, 15, 30, and 60 min. This was done in males and in females and I would like to keep in mind sex differences.
My question: do the changes we see in hormones over time differ between groups A,B, and C? And if so, how?
Does the variation of hormone change over time differ between groups A,B, and C?
I would like to analyze in one big model, but I'm not exactly confident in the test(s) I'm using. Any advice?