I have two groups I want to compare, however, I only have one data point in the first group compared to 450 data points in the second group.
Here's the story. I have a patient that has a mutation in one of his miRNA, and I'm trying to find if it affects the expression of the miRNA's target genes. So, I took one of the target genes and I compared its FPKM value (normalised expression) (group 1) to the genes that are expressed in patients that don't have this mutation (group 2).
I thought about doing a T-test, but obviously can't perform a T-test with what I have. Do I need to generate "artificial" data in my first group in accordance with the elements from my second group? Is there a package in R or a tool that could help me generate this data? I tried searching for solutions but I only found options for re-sampling already existing data or creating new random data, which I do not want to do.
Thank you for your help!