Here a R solution with a Wilcoxon test and a figure example: ``` r # library library(tidyverse) library(ggpubr) # get data sample= c(1:30) M1=c(3,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,2,3,3,3,3,3,2,2) M2=c(3,2,3,3,2,2,3,3,3,2,2,3,2,2,2,2,2,2,2,2,2,2,2,3,3,3,3,3,2,2) data.frame(sample,M1,M2) -> df # calculate wilcox test wilcox.test(df$M1,df$M2) #> Wilcoxon rank sum test with continuity correction #> #> data: df$M1 and df$M2 #> W = 360, p-value = 0.09554 #> alternative hypothesis: true location shift is not equal to 0 # prepare data for figure df %>% gather(key="key",value="value",-sample) -> df # make figure my_comparisons <- list( c("M1", "M2")) ggboxplot(df, x = "key", y = "value", color = "key", palette = "jco")+ stat_compare_means(comparisons = my_comparisons) # Add pairwise comparisons p-value [![figure][1]][1] [1]: https://i.sstatic.net/htsGg.png