I am analysing data by means of multiple regression. My goal is to find out about the relative importance of the independent variables, using hierarchical partitioning (package `hier.part` in `R`). However, I assume that the interaction of some of the independent variables is important, too. Now I am wondering if it is valid to have interactions when doing hierarchical partitioning? I could not find anything in the literature ([Mac Nally (2002) - Multiple regression and inference in ecology and conservation biology: further comments on identifying important predictor variables][1]) My model would look something like this: DV ~ IV1 + IV2 + IV1:IV2 + IV3 + IV4 + IV5 Result of hierarchical partioning with interaction: I IV1 20.8255247 IV2 4.3218387 IV1:IV2 70.7574155 IV3 1.6795456 IV4 0.8780111 IV5 1.5376644 Result of hierarchical partioning without interaction: I IV1 74.132474 IV2 14.690646 IV3 6.872467 IV4 1.311382 IV5 2.993031 [1]: http://link.springer.com/article/10.1023/A:1016250716679