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ANOVA stands for ANalysis Of VAriance, a statistical model and set of procedures for comparing multiple group means. The independent variables in an ANOVA model are categorical, but an ANOVA table can be used to test continuous variables as well.

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Can repeated measures ANOVA be used with only 2 time points?

In short, yes a repeated measures ANOVA is possible with 2 time points. … A 3-way ANOVA is not the analysis you want, because unlike your variable "day", the variables temperature and salinity are not within-subjects. …
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