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Corrected some minor typos.

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Your general understanding of one sample ttestst-tests is correct. I havn'thaven't studied biology since high school, but a few thoughts...

The antibodies and replicates are somehow the same, and the t-test is a paired t-test between each treated and untreated similar 'thing', and a paired t-test is a one sample t-test.
In a lot of biological work, I know multiple hypothesis testing is a big thing, and when trying to decide what to even analyze, instead of analyzing a million things, you only keep those that have significant signals, so. So maybe that one-sample t-test is referring to some part of the process where they decide which signals to keep by just comparing the signal effect to some mean (and thus each is a one sample ttestt-test?)
It's in the supplementary methods, so could be a typo or a mistake. If you understand the biology and that doesn't make sense, and it's not 1 or 2, I would email the authors and ask! There's nothing wrong with doing that!

Your general understanding of one sample ttests is correct. I havn't studied biology since high school, but a few thoughts...

The antibodies and replicates are somehow the same, and the t-test is a paired t-test between each treated and untreated similar 'thing', and a paired t-test is a one sample t-test.
In a lot of biological work, I know multiple hypothesis testing is a big thing, and when trying to decide what to even analyze, instead of analyzing a million things, you only keep those that have significant signals, so maybe that one-sample t-test is referring to some part of the process where they decide which signals to keep by just comparing the signal effect to some mean (and thus each is a one sample ttest?)
It's in the supplementary methods, so could be a typo or a mistake. If you understand the biology and that doesn't make sense, and it's not 1 or 2, I would email the authors and ask! There's nothing wrong with doing that!

Your general understanding of one sample t-tests is correct. I haven't studied biology since high school, but a few thoughts...

The antibodies and replicates are somehow the same, and the t-test is a paired t-test between each treated and untreated similar 'thing', and a paired t-test is a one sample t-test.
In a lot of biological work I know multiple hypothesis testing is a big thing, and when trying to decide what to even analyze, instead of analyzing a million things, you only keep those that have significant signals. So maybe that one-sample t-test is referring to some part of the process where they decide which signals to keep by just comparing the signal effect to some mean (and thus each is a one sample t-test?)
It's in the supplementary methods, so could be a typo or a mistake. If you understand the biology and that doesn't make sense, and it's not 1 or 2, I would email the authors and ask! There's nothing wrong with doing that!

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created Jun 5, 2020 at 17:39

Your general understanding of one sample ttests is correct. I havn't studied biology since high school, but a few thoughts...

The antibodies and replicates are somehow the same, and the t-test is a paired t-test between each treated and untreated similar 'thing', and a paired t-test is a one sample t-test.
In a lot of biological work, I know multiple hypothesis testing is a big thing, and when trying to decide what to even analyze, instead of analyzing a million things, you only keep those that have significant signals, so maybe that one-sample t-test is referring to some part of the process where they decide which signals to keep by just comparing the signal effect to some mean (and thus each is a one sample ttest?)
It's in the supplementary methods, so could be a typo or a mistake. If you understand the biology and that doesn't make sense, and it's not 1 or 2, I would email the authors and ask! There's nothing wrong with doing that!