Thank you so much. I am going to rethink my design again!

@EdM I have two stratas. I have total 54 deaths and 298 alive in the whole dataset. The measurment was at different time points by RNA-seq, I have to do normalization then split by the median. So, all the genes are actually 0 for below median and 1 for above the median.

@BruceET thank you for your answer. But do you mean that I should not mix them! I think I need to get more deep to understand on what basis Markov chain is looking at one event!

Please specify if all of these groups are related or you are trying to get mean difference between each individual group and its control