I test the effect of a plant dose on the abundance of a specific microbe, cultured in glass tube in vitro. The treatment consist of 5 doses (0, 1, 2, 3, 4). Each culture tube receives one of the dose in 4 replications. The abundance of the microbe is observed after two times point: 12h and 24h. I would like to check how the plant dose affect the microbe abundance throughout the time. Is this a randomized complete design with two repeated measures, a randomized complete block design with time point considered as block? or a 4 X 2 factorial design? I am confused between those three experimental design.