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Alan
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` I had forgotten to include my model's summary in my my question, so here it is:

> summary(lmm.1)
Linear mixed model fit by REML ['lmerMod']
Formula: condition ~ category.of.urbanization + (1 | river)
Data: fish

REML criterion at convergence: -214.3

Scaled residuals: 
Min       1Q   Median       3Q      Max 
-2.65967 -0.54776 -0.07734  0.56748  2.79995 

Random effects:
Groups   Name        Variance Std.Dev.
river    (Intercept) 0.001965 0.04432 
Residual             0.012381 0.11127 
Number of obs: 151, groups:  river, 6

Fixed effects:
                                 Estimate Std. Error t value
(Intercept)                      -0.12808    0.04154  -3.084
category.of.urbanizationslightly  0.15972    0.05376   2.971
category.of.urbanizationvery      0.17063    0.05432   3.141

Correlation of Fixed Effects:
              (Intr) ctgr.d.rbnzcp
ctgr.d.rbnzcp -0.773              
ctgr.d.rbnzcm -0.765  0.591    

`

` I had forgotten to include my model's summary in my my question, so here it is:

> summary(lmm.1)
Linear mixed model fit by REML ['lmerMod']
Formula: condition ~ category.of.urbanization + (1 | river)
Data: fish

REML criterion at convergence: -214.3

Scaled residuals: 
Min       1Q   Median       3Q      Max 
-2.65967 -0.54776 -0.07734  0.56748  2.79995 

Random effects:
Groups   Name        Variance Std.Dev.
river    (Intercept) 0.001965 0.04432 
Residual             0.012381 0.11127 
Number of obs: 151, groups:  river, 6

Fixed effects:
                                 Estimate Std. Error t value
(Intercept)                      -0.12808    0.04154  -3.084
category.of.urbanizationslightly  0.15972    0.05376   2.971
category.of.urbanizationvery      0.17063    0.05432   3.141

Correlation of Fixed Effects:
              (Intr) ctgr.d.rbnzcp
ctgr.d.rbnzcp -0.773              
ctgr.d.rbnzcm -0.765  0.591    
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Alan
Alan

If the categorical variable is retained in my final model in R, then why does the post hoc analysis say the levels do not differ?

I am performing model selection in R with the anova() function, and my categorical variable was maintained in my final model, but when I did a post hoc analysis with the emmeans() function, it told me the levels did not differ. What does it mean?

I use R software, and I am studying how the body condition of a species of fish varies in 3 kinds of rivers: preserved, slightly urban and very urban. Each category has one replicate, so that means I have 2 preserved rivers, 2 slightly urbanized rivers and 2 very urbanized rivers, which means that "river" is a random factor, and "category of urbanization" is my fixed factor and predictor variable with 3 levels. While performing model selection in R with the anova() function, the categorical variable "category" is maintained:

`#it is a linear mixed model because condition is normally distributed
> lmm.1 <- lmer(condition ~ category.of.urbanization + (1|river), data = fish) 
> lmm.null <- lmer(condition ~ 1 + (1|river), data = fish) 
> anova(lmm.null, lmm.1)
refitting model(s) with ML (instead of REML)
Data: fish
Models:
lmm.null: condition ~ 1 + (1 | river)
lmm.1: condition ~ category.of.urbanization + (1 | river)
            npar     AIC     BIC logLik deviance  Chisq Df Pr(>Chisq)   
lmm.null       3 -214.42 -205.37 110.21  -220.42                        
lmm.1          5 -219.80 -204.71 114.90  -229.80 9.3806  2   0.009184 **
---
Signif. codes:  0 ‘***’ 0.001 ‘**’ 0.01 ‘*’ 0.05 ‘.’ 0.1 ‘ ’ 1`

`

My p-value is 0.009184, meaning category of urbanization is an important predictor, and I expected that at least one level of the categorical variable would be different from the others. However, when trying to do a post hoc analysis, I called the emmeans() function, and R says that none of the levels differ, because the p-values are all above 0.05:

`> emmeans(lmm.1, pairwise ~ category.of.urbanization)
Registered S3 methods overwritten by 'broom':
method            from  
tidy.glht         jtools
tidy.summary.glht jtools
$emmeans
category.of.urbanization  emmean     SE   df lower.CL upper.CL
preserved                -0.1281 0.0441 3.42  -0.2592  0.00304
slightly urban            0.0316 0.0341 2.20  -0.1030  0.16632
very urban                0.0425 0.0350 2.43  -0.0852  0.17032

Degrees-of-freedom method: kenward-roger 
Confidence level used: 0.95 

$contrasts
contrast                    estimate     SE   df t.ratio p.value
preserved - slightly urban   -0.1597 0.0558 2.83  -2.863  0.1324
preserved - very urban       -0.1706 0.0563 2.95  -3.028  0.1123
slightly urban - very urban  -0.0109 0.0489 2.32  -0.223  0.9733

Degrees-of-freedom method: kenward-roger 
P value adjustment: tukey method for comparing a family of 3 estimates `

Please, what does this mean? How is the predictor variable significant, but with levels that aren't different? I have 151 fish, so my number of data and observations is not very low. I am sorry if I've made spelling mistakes, English is not my native language.