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Statistical significance is a characteristic of a statistic viewed in light of a null hypothesis and a given significance level. It reflects whether the statistic belongs to the rejection region (is statistically significant) or the acceptance region (is not statistically significant).

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Statistical Test for Many different groups before and after an Event?

This would be most suitable as a comment but I don't have comment privileges yet. Do you want to look at how each species reacts to the medicine? If not, you can just look at the overall before and af …
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