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I am doing DNA extractions and DNA quality assesment on those results, based on spectrometry (260/280nm values). So i want to compare or see if there is differences between the DNA extracted from leaves v/s buds from the same tree, with 15 individuals.

I think that t-test is one of the approaches that i can use, but i'm struggling choosing between paired and independent t-test. I could be wrong in thinking that t-test is the approach, so i appreciate any suggestions

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  • $\begingroup$ It will help to describe the data you collect in more detail. How many data points (extractions & quality assessments) do you from each tree? $\endgroup$
    – dipetkov
    Commented Jun 16 at 15:39

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Welcome to Cross Validated! Because your group A (leaves) and group B (buds) measurements are being collected from the same trees, a paired test (either paired t-test or Wilcoxon signed rank test) would be best to use.

You should also descriptively look at your data using visualizations such as a histogram, box plot, etc. to get an idea of the distribution of your outcome variable. This will help inform which test to use.

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